Abstract: The effect of low frequency pulsed ultrasound on the recovery of skeletal muscle fatigue is studied by establishing the exercise fatigue model in rats. The correlation between low frequency pulsed ultrasound and recovery of skeletal muscle fatigue in rats is investigated by analyzing physical (weight) and biochemical (CK, BUN, MDA, CRE and calcium) indexes of muscle tissue. The results show that the CK value and BUN value in the serum of the rats are more than those in the fatigue group (FG) after ultrasonic irradiation, and the body weight increases in a certain range. Preliminary experiments show that low-frequency pulsed ultrasound has a certain promoting effect on reliving muscle fatigue.