Abstract: Based on the theoretical assertion that low-frequency noise has a significant influence on people, an experimental evaluation of subjects is conducted by using the graphic rating scale method. The hypothesis of this test is that low-frequency sound could modulate people’s emotional response and subjective perception. The multifrequency sound and the narrowband signals of 1/3 Octave range, which are all at low frequencies are used as the test signals. The obtained results show that low-frequency sound could enhance subjective emotional perceptions, such as elevating arousal levels, causing subjects to feel overwhelmed by the perceived “domineering” effect of the sound, and causing intensified sensations of annoyance as well as altered perceptions of space and immersion. This scientific discovery could be applied to enhancing and manipulating the emotion experience of sensory media such as film, through the strategic use of low-frequency sound.