Abstract: Solution crystallization technology is widely used in many fields and its crucial aspect is nucleation. Dual frequency ultrasonic irradiation (25 kHz+40 kHz) is proposed to enhance the nucleation of anhydrous glucose solution. The effects of the concentration of the solution, the ultrasonic power and ultrasonic processing time on the nucleation rate are studied. The crystal size under the dual frequency ultrasonic irradiation is analyzed by comparison with the single frequency ultrasonic irradiation. And the iodine released from potassium iodide is determined to assess the cavitation yields of dual and single frequency ultrasound. The results show that the dual frequency ultrasound could, under the same condition, reduce the initial concentration of the nucleation of anhydrous glucose solution, increase the nucleation rate and achieve the crystal nucleus, which has regular morphology and uniform particle size distribution by comparison with no ultrasound and with single frequency ultrasound. The results of absorbency experiments indicate that the cavitation yields, i.e. the iodine content, of dual frequency ultrasound is more than that of a single 25 kHz or 40kHz ultrasound, that is to say, a significant synergetic effect of dual frequency ultrasound exists. Consequently, nucleation under the dual frequency ultrasonic irradiation is a rapid, high-effective and energy-saving method.