Abstract: The article illustrates the harm of marine biofouling and the characteristics of marine organism. Also, comparisons between traditional anti-fouling techniques and methods are made in this paper, including physical methods and chemical methods. But, there are some defects in these traditional methods, such as high strength labor, high cost and environmental pollution. This article introduces the new ultrasonic anti-fouling technique. However, up to now very few researches on using ultrasonic to treat marine biofouling have been reported. In this article, a set of experimental scheme is designed for separately doing the static and dynamic tests to kill the larvae and ova of marine organism. The test results show that the effect of ultrasonic wave on killing marine biofouling is obvious, and this technique has several advantages in solving biofouling problem, such as easy operation, low cost, high efficiency and environmental protection. Thus the ultrasonic anti-biofouling technique has a broad application prospect in marine anti-biofouling.