Abstract: In order to explore the vocal characteristics and regularity of Penaeus vannamei feeding, the experiment is carried out under the environment of cultured shrimp pond, and the hydrophone and collector are used for acquiring signal. The spectrum analysis and time-frequency analysis of the signal are performed by Matlab software to obtain the regularity of Penaeus vannamei vocalization. Experiments show that in the freshwater aquaculture environment, the acoustic signals produced by Penaeus vannamei have certain regularity. The duration of a single pulse signal is 0.005 s and the frequency of the main peak is between 2~10 kHz. After feeding bait, the main peak frequency is expanded to about 5~15 kHz and the signals produced by the Penaeus vannamei increase with the spread of chewing activity, which makes the time-frequency domain characteristics of the signal significantly different before and after feeding. The result of this experiment shows the possibility of using the signal generated by Penaeus vannamei to control the feeding machine for on-demand feeding.