Abstract: Aiming at the requirement of acoustic characteristic test of ship wake bubbles, the development of test system prototype and the study of actual navigation test are carried out. The basic principles of acoustic detection and inversion of ship wake geometrical size and bubble distribution density are described. Based on the comprehensive analysis of domestic research status, the remote operated vehicle (ROV) platform based mobile integrated test scheme is adopted to solve the test problems at different water depths, and the comparative analysis method is used to reduce the influence of underwater acoustic environment on the acoustic measurement system for the ship wake bubbles. The corresponding test system is developed and the actual navigation test on lake is performed. The test results are basically consistent with the current research and test findings, which proves that the test system developed in this paper has the capability of acoustic detection of ship wake bubbles, and has a wide range of military and civil values.