Abstract: The biological effect of ultrasound has always been widely concerned. Particularly, the mechanism of low-intensity ultrasound (LIUS) on cells has also been continuously investigated and understood. In order to study the effect of LIUS on the development of zebrafish embryos, the resonant frequencies of spherical and ellipsoidal cells are obtained by acoustic resonance theory and finite element simulation. Therefore, an ultrasonic stimulation device is designed according to the frequencies calculated to stimulate the zebrafish embryos. The effects of three parameters, stimulation frequency, stimulation time and excitation voltage, on the hatching index of fish eggs are studied through orthogonal experiment design. The results show that the hatching index of fish eggs is significantly higher than that of the control group under the conditions of frequency 1.26 MHz, stimulation time 30 min and the signal excitation voltage 3Vpp, and the optimized stimulation frequency is basically consistent with the simulation result. Therefore, it can be concluded that the results of theoretical derivation and simulation calculation are reliable, and the LIUS stimulation can possibly accelerate the growth of zebrafish embryonic cells.