Abstract: By using 1,3-diphenylisobenzofuran (DPBF),the activating efficiency of hematoporphyrin (HPD) by ultrasound has been detected and the relations of acoustic parameters with the activating efficiency have been found.The results show that under a certain condition, the relative loss of DPBF will rise up with the increasing of insonation time and acoustic intensity.Moreover the acoustic frequency and HPD concentration have influenced on the relative loss of DPBF.The result is of great significance for driving sonodynamic treatment to clinic application.