Abstract: A new ultrasonic apparatus used for enhancement of supercritical fluid extraction(SFE) was designed, and the ultrasound,enhanced SFE process studied. Coix lacryma-jobi seed oil, coix lacryma-jobi seed ester, EPA and DHA were extracted from coix lacryma,jobi seed and algae, respectively with this ultrasound,enhanced SFE process. The results show that the apparatus reduced not only extraction pressure, temperature and time, but also the supercritical flow rate and the modifier usage. It improved the extraction efficiency remarkably. The results of GC-MS analyzer indicate that the components and their structure do not change under ultrasound irradiation.