Abstract: A real-time high-accuracy three-dimensional localization of sound source system is designed ba-sed on AT89S52 single-chip microcomputer. It is applicable to detecting the aural signal of low frequency ranging from 20Hz to 2000Hz. It is mainly composed of three modules: sound source gathering and analog-to-digital conversion module,time delay gathering module and serial communication and algorithm module. In the region of 1000m³,the three-dimensional position of sound source can be localized rapidly in real-time. The maximum positional errors are ±0.3 degree in horizontal direction and ±0.5 degree in vertical direction.