Abstract: seafloor sediment samples must be carried to Laboratory for indoor acoustical and physical parameter measurements after keeping and transporting the samples pick up from seafloor.In this course,the samples would unavoidably encounter various interferences.Making artificial samples in laboratory and simulating the interference that the samples encountered,the relationship between acoustic attenuation and disturbed water content can be examined.Through spectrum analyses,it can be concluded that the random perturbation is difficult to distinguish and the mistaken test result is apt to make.The possible perturbation is analyzed and some suggestion on experimental tests is put forward.